Other

Part:BBa_K4430009:Design

Designed by: Ruyi Shi   Group: iGEM22_GYHS   (2022-09-20)


TFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1432
    Illegal AgeI site found at 1087
    Illegal AgeI site found at 1525
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 526


Design Notes

Researchers had shown that TFP binds strongly to the cell surfaces of E. coli strains. In order to separate EC from food samples, TFP was coupled with carboxyl magnetic beads, and then EC could be separated from the samples through magnets. At last, EC was detected combined with paper-based sensor.


Source

The source of the part will be its sequence which was retrieved from NCBI.

References